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Bacterial Outer Membrane Protein Assembly and Cell Surface DisplayPosted by: National Institutes of Health (NIH)
Posted date: 2018-Jul-06
A post-doctoral position is available to investigate the assembly of bacterial outer membrane proteins in Gram-negative bacteria. While most integral membrane proteins contain one or more α-helical membrane spanning segments, these proteins contain a “β barrel” domain that serves as a membrane anchor. In many of our experiments we have been investigating the assembly of “autotransporters”, a large superfamily of outer membrane proteins produced by pathogenic bacteria that contain a large extracellular domain ("passenger domain") as well as a b barrel domain. Autotransporters are of particular interest both because they are virulence factors and because the mechanism of passenger domain secretion is unknown. We are currently using a combination of biochemical, genetic and structural approaches to investigate the role of a variety of periplasmic and outer membrane factors in promoting both the secretion of the passenger domain and the membrane integration of the b barrel domain. We are also interested in developing methods to use autotransporters as a tool to display heterologous polypeptides on the bacterial cell surface or to secrete them into the extracellular milieu. These methods may be useful for vaccine development and for other biotechnology applications.
For examples of our previous work on autotransporter/outer membrane protein assembly, please see Barnard, T.J. et al. (2007) Nat. Struct. Mol. Biol. 14: 1214-1220; Ieva, R. and Bernstein. H.D. (2009) PNAS 106: 19120-19125; Peterson, J.H., et al. (2010) PNAS 107: 17739-17744; Ieva, R. et al. (2011) PNAS 108: E383-E391; Pavlova, O. et al. (2013) PNAS 110: E938-E947; Roman-Hernandez, G. et al. (2014) eLife e04234; Sikdar, R., et al. (2017) Nat. Commun. 8:1309; Hussain, S. and Bernstein, H.D. (2018) J. Biol. Chem. 293: 2959-2973. Additional information can also be found at http://www.niddk.nih.gov/about-niddk/staff-directory/intramural/harris-bernstein/pages/research-summary.aspx.
The position is located on the main campus of the NIH in Bethesda, MD.Qualifications:
Candidates should have a Ph.D. and/or M.D. degree (or anticipate receiving their degree in the near future) and experience in molecular biology, biochemistry, genetics, microbiology or a related field. Candidates with less than 5 years of post-doctoral experience are preferred.To Apply:
Interested individuals should email their CV, a cover letter summarizing current and future research interests, and contact information for three references to:
Dr. Harris Bernstein
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