Posted by: National Institutes of Health (NIH)
Posted date: 2018-Jul-13
We seek motivated postdoctoral fellows interested in pursuing one of the following two
areas of research at Carnegie Institution’s Embryology Department in Baltimore.
1. The role of genome organization in development, tissue function, and aging.
How cells in different lineages acquire and maintain their unique genome architecture has
remained poorly understood. We use various tools and organisms to study how genome
organization in different cells influences lineage specification and terminal differentiation, how
such organization is maintained in differentiated cells, and whether genome dis-organization
accompanies aging and contributes to organ decay.
a. Kim Y...Zheng Y (2011). Mouse ES cells do not need any lamins but proper organogenesis
requires lamin-Bs. Science 334:1706-1710.
b. Jia J…Zheng Y (2012). Regulation of pluripotency and self-renewal of ES cells through
epigenetic threshold modulation and mRNA pruning. Cell 151:576–589.
c. Chen H, Chen X, and Zheng Y (2013). The nuclear lamina regulates germline stem cell niche
organization via modulation of EGFR signaling. Cell Stem Cell 13:73-86.
d. Chen H, Zheng X, and Zheng Y (2014). Age-associated loss of lamin-B leads to systemic
inflammation and gut hyperplasia. Cell 159:829-843.
e. Zheng X...Zheng Y (2018). Lamins organize the global three-dimensional genome from the
nuclear periphery. Molecular Cell DOI: https://doi.org/10.1016/j.molcel.2018.05.017
2. Cell division mechanisms
We are interested in understanding how cells segregate chromosomes equally into
daughter cells using mitotic spindle apparatus. We discovered protein complexes called γ-tubulin
ring complex (γTuRC) and γ-tubulin small complex (γTuSC) that mediate microtubule
nucleation and organization in mitotic and interphase cells. By using the powerful Xenopus egg
extract system, we and others have uncovered an important signaling pathway mediated by the
nuclear small GTPase Ran that regulates multiple aspects of cell division. More recently we
discovered a new spindle assembly factor called BuGZ that regulates mitosis in part by its ability
to undergo protein phase separation or transition.
a. Zheng Y…Mitchison TJ (1995). A γ-tubulin ring complex from the unfertilized egg
of Xenopus laevis can nucleate microtubule assembly in vitro. Nature 378:578-583.
b. Wilde A & Zheng Y (1999). Stimulation of microtubule aster formation and spindle assembly
in Xenopus egg extracts by the small GTPase Ran. Science 284:1359-1362.
c. Wiese C…Zheng Y (2001). Role of importin-b coupling Ran to downstream targets in
microtubule assembly. Science 291:653-656.
d. Tsai M-Y…Zheng Y (2006). A mitotic lamin B matrix induced by RanGTP required for
spindle assembly. Science 311:1887-1893.
e. Jiang H…Zheng Y (2014). A microtubule-associate zinc finger protein, BuGZ, regulated
mitotic chromosome alignment by ensuring Bub3 stability and kinetochore
targeting. Developmental Cell 28:268-281.
f. Jiang H…Zheng Y (2015). Phase transition of spindle-associated protein regulate spindle
apparatus assembly. Cell 163:108-122.
Please contact Dr. Yixian Zheng: zhengcarnegiescience.edu