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Respiratory BiologyPosted by: Postdoc positions by NIH
Posted date: 2019-Jan-23
The Preciado laboratory at Children's National Medical Center is part of the Division of Pediatric Otolaryngology. The NIH-funded research lab investigates the molecular mechanisms of mucin gene regulation in otitis media progression. In collaboration with the Airway Epithelial Biology group at Children’s National they have developed extensive expertise culturing upper airway primary epithelium using reconditioned growth media with ROCK inhibitors, purifying exosomes from middle ear cells, stable and transient transfection of respiratory epithelium, and in vivo models of middle ear infection. The lab’s preliminary and published work has investigated the role of MUC5B along OM continuum, as his group was the first to describe MUC5B as being the predominant mucin protein in middle ear fluid by using an unbiased mass spectrometry approach. Additionally, key previous work reported that MEEs also contain exosomes, nanovesicles implicated in cell-to-cell communication through the microRNAs they carry, exerting a vital role in a variety of epithelial developmental processes, diseases, and infection mechanisms. Of interest, they were the first group to comprehensively characterize middle ear exosomal miRNA content both in vivo and in vitro. Notably, they found that middle ear NTHi exposure results in a direct increase of exosomal packaging heterogenous nuclear ribonuclear proteins (hnRNPs), as well as in the potent induction of a select group of miRNAs, with miR-378 being the most induced miRNA both in MEE from patients and from middle ear cells in culture.
The lab is looking for a postdoctoral reserach fellow, who would be responsible for generating and testing scientific hypothesis related to:
M.S. or Ph.D. degree in laboratory sciences (molecular biology, cell biology, immunology, biochemistry, genetic, etc.). Successful applicant must have ability to think, perform independently, troubleshoot protocols, interact in a small working group. He/she must be well-organized and fluent in spoken and written English.Essential Functions:
Must have hands-on experience with gene regulation and gene expression and the ability to design and carry out experiments that utilize molecular and cell biology techniques.To Apply:
For more information and to submit an application package, please contact: Dedi Anibaba at aanibabachildrensnational.org